This new method can help diagnose Lyme disease in 15 minutes

Researchers have developed a new treatment method capable of detecting Lyme disease in just 15 minutes.

By: ANI | | Published: October 14, 2019 8:46 am

Lyme disease quick diagnosis possible with a new found method. © Shutterstock

Caused by Borrelia burgdorferi and transmitted by the bite of infected Ixodes ticks, Lyme disease if left untreated can cause serious neurologic, cardiac, and/or rheumatologic complications. Also Read - Drug-duo can potentially speed up bone healing process, find scientists

“Our findings are the first to demonstrate that Lyme disease diagnosis can be carried out in a microfluidic format that can provide rapid quantitative results,” said Sam Sia, professor of biomedical engineering at Columbia Engineering. Also Read - Are you suffering from rheumatoid arthritis? Try these gentle workouts

“This means that our test could easily be used directly in a doctor’s office, obviating having to send the samples out to a laboratory that needs at least a couple of hours, if not days, to get test results,” added Sia whose lab is focused on using microfluidics to build low-cost, integrated devices for performing sophisticated medical tests, together with developing new treatment modalities based on cell therapy and implantable devices. Also Read - Diagnosing rheumatoid arthritis to get easier soon

Sia’s group worked in collaboration with Maria Gomes-Solecki from Immuno Technologies, which found a combination of three proteins that identified antibodies specific to the B. burgdorferi bacterium in the serum, and OPKO Health, which provided microfluidic cassettes. Their findings were published today in the journal of ‘Clinical Microbiology’.

The researchers evaluated 142 samples, including patients with early Lyme disease, healthy individuals from areas where Lyme disease is endemic, and those with Lyme arthritis.

They first screened a set of known diagnostic Lyme disease biomarkers for their ability to detect Lyme disease infection. They then tested the top three biomarkers using a standard enzyme immunoassay, and then mChip-LD, an advanced microfluidic platform developed by Sam Sia, to test the samples.

When tested against additional samples of serum from people with Lyme disease, the multiplexed set of biomarkers was more sensitive than standard Lyme disease tests, while also exhibiting high specificity.

The team found that it was better at picking up signs of Lyme disease infection in early-stage samples — possibly because it was able to detect antibodies that peak in the first weeks after someone is infected with Lyme disease.

When the test was run on Sia’s mChip-LD platform, it worked very well, showing strong potential for the development of a point-of-care test for Lyme disease.

“While the assay will require more refinement and testing before it can be approved for widespread use as a test for Lyme disease, our results are very exciting,” said one of the study’s lead authors, Siddarth Arumugam, who is a PhD student in Sia’s lab.

“It will help so many people if we can develop a single, rapid, multiplexed diagnostic test to identify Lyme disease stage that can be used in doctors’ offices,” added Siddarth Arumugam.

Published : October 14, 2019 8:46 am

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